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KMID : 0380619920240030215
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Korean Journal of Food Science and Technology
1992 Volume.24 No. 3 p.215 ~ p.220
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Isolation and Characterization of Caffeine Degrading Bacteria
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Abstract
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Several bacterial strains capable of degrading caffeine were isolated and studied for their biodegradation ability of the caffeine and some biochemical characteristics. The isolate KS-5 was identified as Pseudomonas putida and was designated as the P. putida KS-5. The optimum conditions were at 30℃, pH 7.0 and 1.0% caffeine. Agarose gel electrophoresis and curing experiment were found that the gene for caffeine degradation was encoded on the plasmid in P. putida KS-5 and that this strain was resistant to several antibiotics.
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